1. The LCP-FRAP Imager emits a photobleaching laser pulse.
2.The pulse bounces off a dichroic mirror, through an excitation filters, off a second dichroic, and objective it then strikes the lipid, bleaching it.
3. The LED shines a light through the condenser and the dichroic/excitation filter/dichroic/objective into protein sample.
4. Images are then processes to determine the recovery time of the photobleached spot of the sample to detemine the protein's mobility.