Accelerating Research with Organoid Disease Models Using Automated Imaging and Dispensing
Adapted from a poster presented by Maryna Panamarova, Lucy Holland, Cameron Collins, Chris Henderson, Francesco Iacoviello, Hema Lingala, Lesley Shirley, Amy Yeung at the Organ Modelling and 3D Cell Culture 2024 Conference in March 2024.
Introduction
The culture of organoid models derived from adult stem cells involves embedding them into a hydrogel that provides essential structural support for their growth and differentiation. Manual plating of organoid-hydrogel suspension becomes increasingly difficult for high-throughput techniques such as screening, high-content imaging, extensive expansion, and quality assurance. The temperature-sensitive nature of most hydrogels necessitates rapid dispensing, precise positioning and exact volume of the matrix droplets to ensure reproducibility and enable maximum growth.
Intestinal and oesophageal tumour organoids were harvested and plated into micro well plates either manually or using an automated liquid dispenser Mantis® (Formulatrix). The organoid culture was then imaged on IncuCyte SX5 (Sartorius) for over 7 days and subjected to the kinetics growth analysis.
Effective automated dispensing using the Mantis® (Formulatrix) in combination with live-cell image analysis on the IncuCyte SX5 (Sartorius) opens up the possibility of running high-scale organoid applications such as small molecule screens, quantitative quality assurance processes, and high-volume expansion experiments.
Acknowledgement:
The research presented in this poster is pre-published work. This poster comes courtesy of the Wellcome Sanger Institute in Hinxton, UK.