for Protein Crystallization
Determine If Your Crystals are Protein or Not
Protein crystallization drops are illuminated with ultraviolet (UV) light and the fluorescence generated from aromatic amino acids like tryptophan are detected to create an image. If no fluorescence is detected within a crystal, then it lacks enough aromatic amino acids to produce a signal and is likely not protein.
The UV imaging option is built into ROCK IMAGER with all several automated features, including automated imaging, extended focus imaging (EFI), and regions of interest (ROIs) that can help you see your crystals better.
The ROCK IMAGER UV imaging solution uses 100% UV-optimized components: UV-grade optics, a UV-sensitive camera, and UV lighting. This optimization is critical for achieving the best image quality and fastest imaging speed possible.
Visualize Microcrystals with High Magnification Objectives
UV-optimized optics provide high-contrast images with the ability to see crystals as small as 2 μm. In the image to the right, individual protein crystal needles 2 μm in width are visualized with UV. The UV, LED and condenser lenses are positioned to maximize the intensity of the UV illumination in order to boost protein fluorescence signal strength. Also, the average RMS contrast (standard deviation of the pixel intensities) is optimized to increase the visibility of fluorescing crystals.
Both fixed and compound zoom options are available for Rock Imager UV. The fixed zoom option is available with one of the lenses listed in the table below. The compound zoom option includes all three lenses on a motorized wheel.
|Objective||Numerical Aperture||Depth of Field (mm)||Field of View (mm)||Pixel Size (µL)|
|2.9x||0.25||0.10||4.1 x 3.3||1.25|
|6.5x||0.25||0.07||1.9 x 1.5||0.567|
|9.7x||0.32||0.05||1.3 x 1.0||0.375|
UV Optical Design that Preserves Visible Image Quality
Dual Light Path Option
The color camera and 12x continuous zoom optics are incompatible with UV light, so colored imaging, continuous zoom and regions of interest are not available if visible and UV are combined into a single path. The FORMULATRIX solution uses two uniquely-optimized and adjacent lightpaths. The sample is moved from one microscope to the other with precise registration between the two images. The dual light path solution also corrects chromatic aberration that would not be possible with a single light path configuration.
Add UV Imaging on a Budget
Single Light Path Option
For a budget-friendly option, a single light path design is available where the visible optics and camera are shared with the UV optics. This option is only available with black and white imaging and fixed objectives.
For the ROCK IMAGER 1, the optics for visible and UV imaging are shared and optimized to yield high-resolution UV images while retaining high optical quality visible images. A simple optical design is utilized to increase the throughput of UV fluorescence allowing for shorter exposure times while imaging. Three high numerical aperture objectives are utilized to image drops at varying magnifications.