Protein Crystallization

Increase Throughput + Reproducibility

Automate Your Protein Crystallization Workflow

Manage the entire protein crystallization experimentation process, from design and dispense to image viewing and analysis all from one software, ROCK MAKER®.

ROCK MAKER is a Laboratory Information Management System (LIMS) that is there every step of the way, with powerful yet easy-to-use tools that help you get the results you need quickly.

 

ROCK MAKER integrates with:

NT8® - Drop Setter

FORMULATOR® - Screen Builder

MANTIS® - Liquid Handler

ROCK IMAGER® - Crystallization Imagers

SONICC® - Nanocrystal Detector

FRAP - LCP Conditions Prescreening

Trusted Worldwide by Labs of All Sizes

Our protein crystallization solutions are trusted in hundreds of laboratories worldwide including at the top 10 leading pharmaceutical companies and at academic research institutes from around the globe.

 

Our clients come from a broad range of scientific study and backgrounds. We have flexible automation and software solutions to fit your budget and workflow, regardless of lab size.

 

Reliable + Reproducible Protein Drop Setting

The NT8® is a fast, nanoliter-volume liquid handler with drop dispensing capability for protein crystallization, including Lipidic Cubic Phase (LCP) experiments. The 8-tip head aspirates and dispenses drops from 10 nL to 1.5 μL.

  • Set up hanging drop, sitting drop, microbatch, in meso Lipidic Cubic Phase (LCP) drops, additive and seeding experiments

 

  • 8-tip head aspirates + dispenses drops from 10 nL to 1.5 μL

 

  • Reusable tips give you the option to dispose tips after each dispense or reuse them to reduce costs + environmental waste

 

  • Proportionally-Controlled Active Humidification minimizes sample evaporation and increases experiment reproducibility

Build Protein Crystallization Screens with Ease

The FORMULATOR® Screen Builder is a next-generation liquid handler designed specifically for protein crystallography. The system uses patented microfluidic technology to dispense up to 34 different ingredients. A 96-nozzle dispensing chip can dispense any volume of any ingredient of any viscosity into any well.

  • Positive displacement chip can dispense volumes down to 200 nL with no upper limit into any microplate type, even VDX/Linbro plates

 

  • Integrated barcode scanner automatically loads experiment and ingredient data while bottle sensors automatically know where ingredients are located

 

  • Reduce your lab’s costs and environmental impact as no consumables are needed

 

  • Closed ingredient containers prevent evaporation, contamination, and noxious fumes from escaping
formulator protein crystallography screen builder

Find the optimal conditions for LCP crystal growth...

without waiting on your crystals.

Prior to setting up protein crystallization experiments, you can quickly screen LCP conditions using Fluorescence Recovery After Photobleaching (FRAP). FRAP is an optical technique that assesses how easily protein is moving about in an LCP drop. If protein is aggregated, or the LCP structure is collapsed, the protein will not diffuse and therefore never crystallize. 

  • Accelerate crystallization methods and rule out suboptimal conditions without having to wait on the crystals growing
  • Automated and user-friendly system requires a few clicks of a button to initiate a complete analysis of your plate
  • All of the results and analyses are integrated into FORMULATRIX' protein crystallography specific software lab management software (ROCK MAKER and ROCK IMAGER).

FRAP was developed in collaboration with Vadim Cherezov (TSRI) and the JCIMPT center led by Ray Stevens and supported by the NIH Common Fund in Structural Biology.

Learn More About Your Protein Drops with Crystal Clear Imaging

ROCK IMAGER® is the most trusted imager for protein crystallography and used by the top 10 pharmaceutical companies and many renowned academic centers worldwide.

Image your drops with multiple imaging technologies allowing you to discover more about your protein drops including Visible Light, UV, Multi-Fluorescence Imaging, FRAP, and SONICC.

  • Various models to fit your budget and workflow to image a single plate up to 940 SBS plates or 1500 LCP plates
  • Seamless integration with ROCK MAKER or your current crystallization software via ROCK IMAGER API
  • Temperature regulation options available for precise temperature control from 30°C down to 4°C
  • Multiple plate options available including SBS, Linbro, Nextal, Terasaki/HLA and LCP plates

Remove the guesswork.

SONICC® finds crystals that other technologies cannot.

The ultimate imaging technology in visualizing protein crystals, Second Order Nonlinear Imaging of Chiral Crystals (SONICC) definitively finds crystals buried in precipitate or submicron crystals naked to the eye. Two powerful technologies, Second Harmonic Generation (SHG) and Ultraviolet Two-Photon Excited Fluorescence (UV-TPEF) are combined together in a completely automated imager to quickly image your high throughput protein crystallization plates and positively identify protein crystals.

  • Crystals appear white against a stark black background, helping you to identify crystals even in murky environments

 

  • Quickly find difficult to see crystals simply by glancing at thumbnails of your plate

 

  • Detect extremely thin crystals, microcrystals <1 μm, and crystals obscured in birefringent LCP

 

  • Definitively distinguish between precipitate and crystals

 

  • Determine which crystals are protein and which are not

Testimonials