Sample Concentration - Diafiltration (Buffer Exchange, Desalting)

Less Time at the Centrifuge, More Time for Research

The µPULSE® - TFF System is a fully automated, walk away system that uses tangential flow filtration (TFF) for sample concentration and diafiltration (buffer exchange, desalting).


Walk-Away System
Set your desired parameters and let the system do 95% of the process automatically until it is time to collect your sample; Remotely monitor via a web-based application.


Permeate flow rate up to 2.5x faster than centrifuges with dead-end filters.


Scalable Process
Whether you are trying to scale up or down, µPULSE allows you to control all the parameters of an industrial TFF, at the research scale.


Wide Range of Operating Volumes
Starting volumes between 1 and 100 mL final volume as low as 0.5 mL. Up to 250 mL of buffer exchange.


Lowest Hold-up Volume in the Industry
Our patent-pending pump design offers just 0.65 mL of hold-up volume. Up to 100% hold-up recovery with automated recovery options.


Easy Maintenance
All components in the fluid path are disposable consumables.


The µPULSE consumable chips can be cleaned in place for re-use up to 300 mL of permeate (sample dependent).

Stop Wasting Time Checking on Samples

Simply enter your starting and final volume with optional diafiltration - The μPULSE® - TFF System takes care of the rest.

See Your Progress

No need to stop your progress to check on samples.

Control from Anywhere

Remote control and monitoring with our web-based app.

Walk-Away System

Enter your parameters and walk away. Get notified when your sample is ready!

Easy Sample Concentration and Diafiltration

(Buffer Exchange, Desalting)

Concentrate Samples Faster with TFF

Unlike centrifuges with traditional dead-end filtration systems, the µPULSE prevents a high concentration gradient from forming at the filtration membrane. This means no reduction in the permeate flow rate and increased sample recovery.


With TFF, the concentration process is continuous and gradual allowing for samples to be concentrated up to 2.5x faster than using dead-end filtration. The exact concentration rate depends on sample size and membrane cutoff size.

tangential flow filtration

The µPULSE utilizes a filtration membrane that is 50 - 75% larger than traditional dead-end filtration systems. This larger membrane boasts a higher membrane flux allowing you to concentrate samples faster so you can move on with your research.

Easy to Maintain with Disposable Filter Chips


The μPULSE - TFF System uses disposable filter chips and transfer tubing. The entire fluid path can be cleaned in place for re-use or discarded for single-use.


Cover a wide variety of sample sizes with 10, 30, 50, and 100 kDa MWCO PES (polyethersulfone) membranes.


5 kDa and 300 kDa coming soon!

"The µPULSE was easy to use right out of the box and fit our process testing needs quite well. It performed comparatively well against our established TFF process and we were able to translate our older process onto the instrument with minimal issues."

- Bijou Bose, GRO Biosciences

GRO-Biosciences-340x179 (2)

Simple Diafiltration

Buffer Exchange, Desalting

Along with concentrating your sample, there is an option for automatic diafiltration to facilitate buffer exchange or desalting processes.


Setup is easy with two 50 mL centrifuge tubes located at the front of the system:


  • One tube holds your sample solution
  • The other a buffer or any other solution of your choice


The system can be refilled with up to 250 mL of buffer for high diavolume exchanges.


Easy Setup - Monitor from Afar

The µPULSE - TFF System utilizes an intuitive, web-based software application that can be run on the device or via external devices on the same network.


This easy-to-use solution allows the user to set up the starting and desired volumes and concentrations, diafiltration or buffer exchange, and modify advanced settings to optimize concentration for your samples.


Users can remotely monitor the process of their concentration instead of having to manually check.