Sample Concentration - Diafiltration (Buffer Exchange, Desalting)
Less Time at the Centrifuge, More Time for Research
The PULSE - TFF System is a fully-automated, true walk away system that uses miniaturized tangential flow filtration (TFF) for sample concentration and diafiltration (buffer exchange, desalting). Gone are the frequent trips to the centrifuge to check on samples and balancing tubes!
True Walk Away System
Set your desired parameters and let the system do the rest. Remotely monitor and control via a web-based application.
Faster Sample Prep
Permeate flow rate is over 2x faster than centrifuges with dead-end filters; go from 20 mL to 1 mL in ~10 mins
Max Working Volume of 100 mL
Working volume is larger than 15 mL dead-end filters, but smaller than standard TFF systems (starting at ~0.5 L)
The system’s filtration chips are reusable up to 3x
Filtration components are disposable; Little-to-no cleaning needed of non-replaceable components
Stop Wasting Time Checking on Samples
Simply, select the original and desired volumes and whether diafiltration is needed. The PULSE - TFF System takes care of the rest.
See Your Progress
No need to stop your progress to check on samples with full visibility of the solutions at all times.
Control from Anywhere
Remote control and monitoring of the progress is available through a web-based app accessible anywhere.
No Need to Balance
Did you check if the centrifuge
Oh wait...There isn't one!
Easy Sample Concentration and Diafiltration (Buffer Exchange, Desalting)
Concentrate Samples Faster with TFF
Unlike centrifuges with traditional dead-end filtration systems, the PULSE - TFF System prevents a high concentration gradient from forming at the filtration membrane. This means no reduction in the permeate flow rate and increased sample recovery.
With TFF, the concentration process is continuous and gradual allowing for samples to be concentrated over 2x faster than using dead-end filtration.
The PULSE - TFF System utilizes a larger filtration membrane (50-75% larger) as compared to traditional dead-end filtration systems. This larger membrane boasts a higher membrane flux allowing you to concentrate samples faster so you can move on with your research.
Easy to Maintain with Disposable Filter Chips
The PULSE - TFF System uses disposable filter chips that make it easy keep the system clean and prevent any cross-contamination.
A built-in diaphragm pump switches between vacuum (-10 - 0 psi) and pressure (40 - 50 psi) to circulate solutions over the concentration membrane.
The chip and source reservoirs are also pressurized (15-20 psi) to help force permeate out and expedite the process.
Three different concentration membranes are available with cutoff sizes of 10kDa, 50kDa, and 100kDa to cover a wide variety of sample sizes.
Buffer Exchange, Desalting
Along with concentrating your sample, there is an option for automatic diafiltration to facilitate buffer exchange or desalting processes.
Set up is easy with two 50 mL centrifuge tubes placed on the front of the system:
- One tube holds your sample solution
- The other a buffer or other solution of your choice
The system can be set in a continuous diafiltration mode or set for discrete diafiltration steps.
The status for the diafiltration can be monitored through the web-based software application.
Control and Monitor from Anywhere
The PULSE - TFF System utilizes an intuitive, web-based software application that can be accessed from anywhere.
This easy-to-use solution allows the user to control the starting and desired volumes and concentrations.
The system also allows for users to define the amount of internal pressure is used during the process to help protect your samples.
Users can track and control the process of their concentration or diafiltration from anywhere with internet access instead of having to manually check.