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Fast Lab-Scale Protein Refolding by Filter Dialysis on the µPULSE TFF System

Application Note

The µPULSE, a lab-scale TFF system, offers customizable pressures for protein refolding, yielding faster and higher specific activity results compared to equilibrium dialysis. This applies broadly to buffer exchange scenarios.

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Practical Aspects of Sample Concentration and Buffer Exchange Utilizing a Miniaturized Tangential Flow Filtration (TFF) System

Application Note

Our TFF system is capable of ultrafiltration rates up to 5 times faster compared to a 15 mL centrifugal device and is completely automated with non-contact liquid sensing, bringing the retentate volume down to the specified setpoint.

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Sample Concentration and Buffer Exchange Utilizing a Miniaturized Tangential Flow Filtration (TFF) System

Application Note

PULSE TFF System completely recover dead volume under one milliliter and can accommodate volumes up to 50 mL if performing diafiltration in the same run, or up to 100 mL if only concentrating. The PULSE TFF System is capable of ultrafiltration rates up to 5 times faster compared to a 15 mL centrifugal device.

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