Cellular viability assays are used to investigate the potency or toxicity of biologically active molecules. One of the widespread techniques to measure cellular viability is a luminescent CellTiter-Glo® (CTG) Assay. It is a homogeneous method based on quantification of the ATP present, an indicator of metabolically active cells. However, CTG tends to dry out and form clogs in multi-time point cell viability studies that require an extended period of incubation.
Cell viability at Time = 72 hours using fresh CTG. The Multidrop® Combi on the left, the Tempest on the right
In the present study, the results of long incubation CTG assays set up with two reagent dispensers, Multidrop® Combi (ThermoFisher) and Tempest® (Formulatrix®), are examined and compared.