Less Time at the Centrifuge, More Time for Research
The aµtoPulse - TFF System is a fully automated, walk-away system that uses Tangential Flow Filtration (TFF) for high-throughput sample concentration and diafiltration (buffer exchange, desalting).

High-throughput
Process up to 54 samples per run and run up to 4 samples in parallel with independent pressure control (configurable to your needs).
Lowest Hold-up Volume, Industry-wide
Starting volume between 0.5 mL and 100 mL. The final volume can be as low as 250 µL with +/-25 µL resolution.
High-flux
With dual diaphragm pumps, the permeate flow rate is up to 1.7x faster than the µPulse and up to 4x faster than centrifugal dead-end filters.
Buffer Flexibility
Use up to 4 different external buffer reservoirs or on-deck buffer conical tubes.
Automatic Cleaning-in-Place
Auto-clean the entire liquid path for every run and auto-rinse with buffer/water to reach the ideal conditions of your sample. The sample fluid path is entirely consumable.
Streamline Your Workflow
The aµtoPulse is a high-throughput walk-away system. Simply set the deck with up to 54 samples and input the process parameters. Labware definitions can be loaded via upstream dispense lists, and protocols can be saved and re-run. The system takes care of the entire process, from cleaning to recovery, and will automatically load the next sample. Remote monitoring of the progress is available through the control software. Eliminate the wasted lab time manually processing and monitoring samples.
Run Versatile Samples
Concentrate Samples Faster, with More Control
Unlike traditional dead-end filtration systems, the aµtoPulse utilizes TFF, which prevents high-concentration gradients from forming on the filter surface. This minimizes the reduction in the permeate flow rate and increases sample recovery. With dual-diaphragm TFF, the concentration process and pressure gradient are near-continuous, imparting less stress on samples than the µPULSE and allowing for samples to be concentrated up to 4x faster than dead-end filtration. Improved back pressure regulation allows transmembrane pressure ranges from 0-32 psi, with independent control and monitoring at each concentration station.
Setup to Match Your Throughput
Max Sample Count per Run
Tube Size (mL) | Concentration Only |
With Buffer Exchange (On Deck Conical) |
With Buffer Exchange (External Reservoir) |
---|---|---|---|
50 | 36 | 24 | 36 |
15 | 48 | 30 | 48 |
1.5 | 54 | 30 | 48 |
Eliminate Cleaning and Cross Contamination
The re-invented disposable filter chips now integrate transfer tubing. An entirely consumable sample path makes it easy to keep the system clean and prevent cross-contamination. The system cleans consumables in place with dedicated cleaning stations linked to external solution reservoirs. Cleaning protocols can be user-defined, including a buffer rinsing to equilibrate the sample flow path to ideal buffer conditions.
Optimize Your Diafiltration Protocol
Along with sample concentration, the aµtoPulse can perform automatic diafiltration to facilitate buffer exchange or desalting processes. External buffer reservoirs allow the option to free up space for more samples; otherwise, the buffers can be loaded via on-deck conical tubes.
Simplify Setup and Monitor Remotely
The aµtoPulse - TFF System utilizes an intuitive, web-based software accessible from anywhere on the network. This user-friendly interface allows scientists to set up even complex protocols, including multi-step concentrations with buffer exchange, and advanced process control settings to optimize concentration for your samples. From here, simply load the deck and press run. Users can remotely monitor the progress of their protocols, eliminating the need for manual observation. Integrating with other lab tools is made easy with CSV import support and runlog exporting.