Developing an immunoassay is challenging. Obtaining a specific signal requires the right combination of sample and detection substrate.

Over the years Cisbio has developed hundreds of assays. In this application note we will show how the MANTIS^® liquid dispenser, with its Large Capacity Chip Changer (LC3) accessory, helped us to improve our process on all of the following key points: reproducibility, time, cost, and reduction of musculoskeletal disorders.

Here we will focus on two types of Homogeneous Time Resolved Fluorescence (HTRF)^® detection assays; biomarker and phospho-protein analysis. HTRF combines the fluorescence sensitivity, time resolution, and low background of TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) with the homogeneous nature of FRET (Fluorescence Resonance Energy Transfer).