Miniaturization of Nextera XT Library Preparation for RNA-sequencing of Murine Neuronal Cell cDNA Samples using the MANTIS® Liquid Handler
Jeremy Lambert1, Tommy Duong2, Paul Scott1, Magnolia Bostick2, Andrew Farmer2
1Formulatrix, Inc., Bedford, MA 2Clontech Laboratories, Mountain View, CA
Miniaturization and scale-down of reagent and reaction volumes for low-input RNA-Seq workflows enables researchers to work with low sample input and increased numbers of samples. As the reaction volumes are reduced, accurate delivery of reagent volumes emerges as a critical step to maintain data quality and technical reproducibility. We present results from miniaturization of the Clontech SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing and Illumina Nextera® XTworkflows for generation of high-quality RNA-Seq samples using MANTIS®, a compact desktop microfluidic dispensing technology.