About
Ultrafiltration and diafiltration (UF/DF) are essential steps in biologics development, enabling protein concentration and buffer exchange prior to final formulation. However, early-stage process development often faces a critical limitation: traditional tangential flow filtration (TFF) systems require sample volumes that simply aren’t available. Scientists are frequently forced to rely on centrifugal devices that are slow, labor-intensive, and prone to sample loss or aggregation.
Even when TFF can be used at small scale, process monitoring typically depends on diavolume estimates and offline assays, providing little visibility into real process behavior. Important effects, such as excipient retention, volume exclusion, or the Donnan effect, can go unnoticed until a formulation drifts off specification.
In this webinar, we introduce a new approach that combines microfluidic tangential flow filtration (µPulse®) with mid-IR spectroscopy (CLADE QA Scan) to enable data-driven UF/DF workflows at lab scale. The µPulse system miniaturizes TFF with a 650 µL hold-up volume, allowing automated, gentle processing of precious samples. When paired with CLADE QA Scan, scientists gain near real-time, multi-attribute monitoring of protein concentration and excipient composition during UF/DF.
Using real experimental data, we will demonstrate how this integrated workflow enables precise monitoring of buffer exchange, protein concentration, and excipient behavior, helping scientists detect process deviations early and make informed formulation decisions while conserving valuable material.
What You’ll Learn
- How microfluidic TFF (µPulse) enables automated ultrafiltration and diafiltration with minimal sample volume
- How near real-time spectroscopy (CLADE QA Scan) can simultaneously monitor protein concentration and formulation excipients
- How to identify non‑ideal process behavior and devise practical strategies to implement data‑driven formulation development
Who Should Attend
This webinar is ideal for:
- Bioprocess development scientists working on UF/DF or TFF workflows
- Formulation scientists developing biologics or monoclonal antibody formulations
- Analytical scientists responsible for process monitoring and characterization
- Process development teams seeking better control of buffer exchange and protein concentration
- Researchers working with limited or precious protein samples
Why Attend?
Learn how to reduce sample consumption, automate UF/DF workflows, and gain near real-time insight into protein formulation processes, all while improving confidence in your development decisions.
When
April 30th 2026
Session 1
10:00 AM - 10:30 AM CEST
(09:00 AM - 09:30 AM BST)
Session 2
01:00 PM - 01:30 PM ET
(10:00 AM - 10:30 AM PT)
About the Speaker:
Dominik Sievert is an accomplished entrepreneur and technology leader with extensive experience in bringing advanced scientific innovations into real-world industrial applications. As the CEO of CLADE, he leads the development of cutting-edge mid-infrared (MIR) spectroscopy solutions that combine proprietary hardware, intelligent software, and analytics to deliver rapid, multi-attribute bioanalytical insights.
About the Speaker:
Muhammad Sajed, PhD, is currently working at Formulatrix as an application scientist for the µPulse-TFF system and its applications in biological research. His areas of expertise include the production and purification of therapeutic enzymes, cell culturing, and industrial biotechnology. Before joining Formulatrix, he held positions as a research officer at the University of Punjab and as a lecturer at Lahore Garrison University.