About

Extracellular vesicles (EVs), particularly exosomes, have emerged as critical mediators of intercellular communication and hold promise for applications in diagnostics, therapeutics, and drug delivery. Harvesting EVs at a lab-scale, however, remains challenging due to the limitations of conventional methods such as ultracentrifugation and dead-end filtration (DEF), which can compromise particle integrity, yield, and functionality.

In this study, we evaluated the µPulse tangential flow filtration (TFF) system for the harvesting of stem cell-derived exosomes. Comparative analyses with DEF units demonstrated that µPulse processing preserved exosome morphology and reduced aggregation, as confirmed by dynamic light scattering and scanning electron microscopy. Exosomes harvested using µPulse exhibited smaller and more uniform sizes and enhanced functionality in wound healing assays, particularly with higher molecular weight cutoff membranes.