Abstract
Full-length single-cell RNA-sequencing (scRNA-seq) is critical for accurately characterizing RNA isoforms, to discover novel cell types, drug development, and personalized therapeutics. scRNA-seq protocols range from plate-based to droplet-based or microwell-based, depending on throughput requirements and the research question. However, the main limitation of traditional plate-based protocols is either providing the artifactual reads while profiling the full-length transcripts or providing only gene-level expression data due to low transcript coverage.
This study highlights the development and optimization of a plate-based method, Single Cell Long-read Isoform Sequencing (scLIS-seq), that delivers high-sensitivity and long-read sequencing while also minimizing artifactual isoform reads. The workflow leveraged 96-channel, positive displacement technology of F.A.S.T.TM (Formulatrix®) liquid handler to precisely automate the error-prone processes like sample dilution, barcode transfer from 96- to 384-well plates, and PCR amplification. This automation was critical for achieving high throughput, time efficiency, and reproducibility.
In this webinar, the scLIS-Seq data will be compared with SMRT-seq3xpress (SS3X) for Jurkat and HE293 to assess RNA isoforms. The webinar will also demonstrate how the scLIS-seq, a new automated method for single-cell RNA sequencing, uses the Formulatrix F.A.S.T. system to accurately capture a critical dimension of the transcriptome: full-length RNA isoforms, opening new doors for discovery.
When
December 18th 2025
Session 1
10:00 AM - 10:30 AM CET
(09:00 AM - 09:30 AM GMT)
Session 1
10:00 AM - 10:30 AM CET
(09:00 AM - 09:30 AM GMT)
About the Speakers:
Dr. Koen Deserranno, University of Ghent, began his career as a Pharmacist and moved into Drug Development in 2021, building expertise in personalized medicine with a focus on genomics, transcriptomics, and pharmacogenomics. He earned his PhD in 2025 on advances in pharmacogenomics and single-cell transcriptomics. He is an active science communicator, contributing to EOS, Nerdland Festival, Dag van de Wetenschap, and DurfDenken.be.
Talha Bin Rahat, the Application Scientist at Formulatrix, holds a master's degree in Healthcare Biotechnology. With a strong background in Forensic Biology, Molecular Biology, Biotechnology, and Virology, he has over three years of lab experience following ISO 17025 principles. Additionally, he has six months of field experience as a crime and death scene investigator.