About
Full-length single-cell RNA-sequencing (scRNA-seq) is critical for accurately characterizing RNA isoforms, to discover novel cell types, drug development, and personalized therapeutics. scRNA-seq protocols range from plate-based to droplet-based or microwell-based, depending on throughput requirements and the research question. However, the current number of strategies compatible with long-read sequencing of the single cell’s transcriptome is minimal, and no direct pairing of the cell’s surface marker expression with its transcriptome is possible.
This study highlights the development and optimization of a plate-based method, Single Cell Long-read Isoform Sequencing (scLIS-seq), that delivers high-sensitivity and long-read sequencing. The workflow leveraged 96-channel, positive displacement technology of F.A.S.T.TM (Formulatrix®) liquid handler to precisely automate the error-prone processes like sample dilution, barcode transfer from 96- to 384-well plates, and PCR amplification. This automation was critical for achieving high throughput, time efficiency, and reproducibility.
In this webinar, the scLIS-seq data will be compared with Smart-seq3xpress (SS3X) for Jurkat and HEK293T cells to assess sensitivity, gene expression, and RNA isoform characterization. The webinar will also demonstrate how scLIS-seq, a new automated method for single-cell RNA sequencing, uses the Formulatrix F.A.S.T. system to accurately capture a critical dimension of the transcriptome: full-length RNA isoforms, opening new doors for discovery.
When
December 18th 2025
Session 1
10:00 AM - 10:30 AM CET
(09:00 AM - 09:30 AM GMT)
Session 1
10:00 AM - 10:30 AM CET
(09:00 AM - 09:30 AM GMT)
About the Speakers:
Dr. Koen Deserranno from the University of Ghent graduated as a Pharmacist specialized in Drug Development in 2021. From there onwards, he started his research career at the Ghent University Laboratory of Pharmaceutical Biotechnology, building expertise in personalized medicine with a focus on genomics, transcriptomics, and pharmacogenomics. In 2025, he earned his Ph.D. on advances in pharmacogenomics and single-cell transcriptomics. Next to his ongoing postdoctoral research, he is an active science communicator, with contributions to EOS and the Nerdland Festival.
Talha Bin Rahat, the Application Scientist at Formulatrix, holds a master's degree in Healthcare Biotechnology. With a strong background in Forensic Biology, Molecular Biology, Biotechnology, and Virology, he has over three years of lab experience following ISO 17025 principles. Additionally, he has six months of field experience as a crime and death scene investigator.