About
Harvesting is a critical step in producing virus-like particles (VLPs), influencing their yield and structural integrity. On a lab-scale, VLP harvesting is conventionally performed using deadend filtration (DEF) units. However, these devices suffer from low filtration rates, high manual intervention, and potential particle damage. In this study, we have evaluated the µPulse, miniaturized tangential flow filtration (TFF) system, for harvesting HIV VLPs. The µPulse demonstrated significantly faster processing times, walk-away operation, and minimal manual handling while maintaining VLP integrity and protein distribution than DEF units.
These advantages make µPulse an effective alternative to DEF units for fast, automated, and cost-effective VLP harvesting, with broad applications in virology and vaccine development.