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Microbial contamination is a persistent issue in cell culture, often affecting experiment reliability and leading to loss of time and resources. This study evaluates the sterility and environmental control of the Cellmatic system, an automated platform designed to support sterile cell culture workflows. Air quality was assessed using passive settle plate sampling at multiple locations within the system, while the sterility of the liquid handling and reagent dispensing was evaluated through automated media exchange and subsequent incubation. Over a multi-month period, no detectable microbial growth was observed in either air or liquid samples.
Uncontaminated (Day 14) vs. contaminated positive control (Day 3)
These results demonstrate the effectiveness of the system’s contamination control strategies, including HEPA filtration, positive pressure, and vaporized hydrogen peroxide–based sterilization. In addition, the system supports routine monitoring through features such as automated agar plate handling and media exchange, allowing users to independently assess environmental conditions with minimal intervention. Overall, the Cellmatic system provides a reliable platform for maintaining aseptic conditions in automated cell culture workflows.