The use of single-cell RNA sequencing has enabled the study of the transcription profiles of individual cells. This provides deeper insights into the conditions of health and disease. Due to the limited source material, it is necessary to use a preparation method with high sensitivity and reproducibility. This application note looks at the integration of the MANTIS^® liquid handler from FORMULATRIX^® into the SMART-Seq^® Single Cell Kit from Takara Bio, enabling miniaturization to one-quarter of the original volume without loss of sensitivity.