Prescreening Membrane Protein

Crystallization Conditions with FRAP

Authors: Lance Ramsey, Ellen Gualtieri, Eric Zhao

 

How can Fluorescence Recovery After Photobleaching (FRAP) save you time?

 

FRAP can be used to determine if a particular crystallization setup has the potential to crystallize. Scientists can check protein constructs, ligands, LCP host lipids and precipitants all in just a 30 minute FRAP assay. Those conditions that show mobility have the potential to then successfully crystallize.