Rapid, Accurate and Flexible DNA Quantitation Using the QuantiFluor® dsDNA System on the MANTIS® Liquid Dispenser

Nucleic acid quantitation is an important step in molecular biology workflows, particularly for advanced applications such as qPCR and NextGeneration Sequencing (NGS). In NGS, the ultimate goal is to generate highly complex libraries sequenced to the greatest degree of depth and uniformity, making accurate quantitation is especially important.

MANTIS App note page (1)

Quantitation of a 2,200bp PCR product used to mimic the average size of a mRNA transcript. The MANTIS liquid dispenser was used to dispense the QuantiFluor® dsDNA System in a 96-well plate format (Panel A) and 384-well plate format (Panel B). Fluorescence was measured on a GloMax® Discover System and unknowns were interpolated from a power regression standard curve.

This application note demonstrates how easy liquid handling software is to use, providing quick and seamless transition to an automated platform. Adding the reagents for the QuantiFluor® dsDNA System using the MANTIS liquid dispenser offers reproducible quantification in multiwall plates with virtually no instrument programming.