Scaling up Organoid Cultures using MANTIS® Liquid Handler: A Case Study with MIT

About Organoid Culture 

Organoids allow researchers to study tissue biology with remarkable fidelity. By providing a three-dimensional in vitro culture system demonstrating key features of a specific organ, these complex, multicellular structures deliver physiologically relevant insight to support many fields of research. A major limitation of conventional organoid culture is that it is often a labor-intensive, high-touch process, requiring highly specialized personnel to deliver only moderate throughput.

Working with MANTIS® 

Using the MANTIS® liquid handler from FORMULATRIX, researchers within the Shalek laboratory at the Massachusetts Institute of Technology (MIT)were able to rapidly screen >450 small molecules at a range of doses for their capacity to enhance differentiation of Paneth cells within small intestinal organoids. Delivering a previously inaccessible level of throughput, the MANTIS® was pivotal to advance understanding of multiple perturbations of limited sample material.

MANTIS® liquid handler
MANTIS® Liquid Handler

MIT researchers have shown the miniaturization approach afforded by the MANTIS® to have proven utility in studying multiple perturbations of limited material. This has relevance to testing organoids derived from a single patient biopsy and opens up many new opportunities for personalized medicine. As technologies evolve, the incorporation of additional modes of measurement will improve model fidelity further still. The MANTIS® is key to enable the greater scale these will demand.