About
The gram-negative bacterial type XI secretion system (TXISS) utilizes surface lipoprotein assembly modulators (SLAMs) to facilitate the secretion of proteins in genus Neisseria. Researchers at the Moraes Laboratory, University of Toronto, have produced the Neisserial SLAMs and surface lipoproteins (SLPs) recombinantly in Escherichia coli for efficient protein secretion and ease of purification. The secreted SLPs were then purified, and crystallization screens were set up using the Formulatrix NT8® Drop Setter and visualized using the Rock Imager® Crystallization Imager.
Figure 1. Gram-negative bacterial translocon system with its substrates
In this webinar, the researchers from the University of Toronto will discuss the potential of SLAM-based secretion systems for versatile protein production and underscore their utility in biophysical and immunological studies. They will also emphasize how the NT8 and Rock Imager facilitated automating their research and data analysis.
About the Speaker:
Dr. Nixon Ng is a Postdoctoral Fellow at the University of Toronto and has expertise in structural biology, protein engineering, computational modeling, and innovative proteomic methodologies. His research focuses on designing a revolutionary class of vaccine antigens targeting medically significant Gram-negative bacterial pathogens. In his current role, he is engaged in optimizing a bacterial surface lipoprotein translocase as an innovative antigen production system.