About
Ultrafiltration and diafiltration (UF/DF) are essential steps in biologics development, enabling protein concentration and buffer exchange prior to final formulation. However, early-stage process development often faces a critical limitation: traditional tangential flow filtration (TFF) systems require sample volumes that simply aren’t available. Scientists are frequently forced to rely on centrifugal devices that are slow, labor-intensive, and prone to sample loss or aggregation.
Even when TFF can be used at small scale, process monitoring typically depends on diavolume estimates and offline assays, providing little visibility into real process behavior. Important effects, such as excipient retention, volume exclusion, or the Donnan effect, can go unnoticed until a formulation drifts off specification.
In this webinar, we introduce a new approach that combines microfluidic tangential flow filtration (µPulse®) with mid-IR spectroscopy (CLADE QA Scan) to enable data-driven UF/DF workflows at lab scale. The µPulse system miniaturizes TFF with a 650 µL hold-up volume, allowing automated, gentle processing of precious samples. When paired with CLADE QA Scan, scientists gain near real-time, multi-attribute monitoring of protein concentration and excipient composition during UF/DF.
Using real experimental data, we will demonstrate how this integrated workflow enables precise monitoring of buffer exchange, protein concentration, and excipient behavior, helping scientists detect process deviations early and make informed formulation decisions while conserving valuable material.
What You’ll Learn
- How microfluidic TFF (µPulse) enables automated ultrafiltration and diafiltration with minimal sample volume
- How near real-time spectroscopy (CLADE QA Scan) can simultaneously monitor protein concentration and formulation excipients
- How to identify non‑ideal process behavior and devise practical strategies to implement data‑driven formulation development
Who Should Attend
This webinar is ideal for:
- Bioprocess development scientists working on UF/DF or TFF workflows
- Formulation scientists developing biologics or monoclonal antibody formulations
- Analytical scientists responsible for process monitoring and characterization
- Process development teams seeking better control of buffer exchange and protein concentration
- Researchers working with limited or precious protein samples
Why Attend?
Learn how to reduce sample consumption, automate UF/DF workflows, and gain near real-time insight into protein formulation processes, all while improving confidence in your development decisions.
About the Speakers
Dominik Sievert is an accomplished entrepreneur and technology leader with extensive experience in bringing advanced scientific innovations into real-world industrial applications. As the CEO of CLADE, he leads the development of cutting-edge mid-infrared (MIR) spectroscopy solutions that combine proprietary hardware, intelligent software, and analytics to deliver rapid, multi-attribute bioanalytical insights.
Greta Jasulaityte is a Sales & Application Specialist at Formulatrix, working with biotech and pharmaceutical customers across Europe, the Middle East, and Africa to integrate the µPulse TFF and aµtoPulse TFF automated filtration platforms into their downstream bioprocessing workflows. She brings over a decade of hands-on expertise in downstream processing, chromatography, and the purification of biologics and gene therapies, giving her a practitioner's perspective on the challenges process teams face every day. Greta holds an EngD in Biochemical Engineering and Bioprocess Leadership from University College London (UCL), with additional MBA coursework completed at London Business School.
Jennifer Mayer is a product manager at CLADE, driving development of the data analytics software Sphere and the app portfolio for biopharma applications. She holds a MSc in Chemistry from the Reutlingen University and specialises in bioanalytics. Building on her scientific background and industry experience, she contributes to advancing CLADE’s mission by helping translate mid-infrared FTIR analytics into easy-to-use tools for formulation development and bioprocessing.