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Generating High-Quality cDNA Libraries for Efficient Sequencing of T-cell Receptor mRNA from Single Cells

Application Note

Single-cell omics offer precise insights into individual cells, including gene expression and variable protein sequences. rhTCRseq enables efficient TCR analysis at single-cell level, supported by MANTIS Liquid Dispenser for quality cDNA libraries.

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Semi-Automated, Non-contact, and Tip-Free Magnetic Bead-based DNA Purification

Application Note

We describe an improved semi-automated workflow using MANTIS in combination with BlueWasher for the purification of nucleic acids that addresses the shortcomings of conventional pipetting.

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Fully Automated, Non-contact, and Tip-Free Magnetic Bead-based DNA Purification

Application Note

Magnetic beads can vary in surface coatings and chemistries for magnetic-based cleanup of DNA, RNA, and proteins with minimal processing. Our presented solution combines micro diaphragm pump technology with centrifugal plate washing to supercharge magbead-based workflows.

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Using the MANTIS Liquid Dispenser to Generate Joint Whole-Genome and mRNA Libraries in Single Cells With DNTR-seq

Application Note

Direct Nuclear Tagmentation and RNA sequencing (DNTR-seq) jointly sequences genomic DNA and mRNA from the same single cell to determine the direct effect of genotype on phenotype. This application note illustrates how the MANTIS liquid dispenser enables multiplex analysis of both DNA and RNA libraries for 1500 cells in a day.

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Accurate Dispensing of Spike-in Control RNA in a Modular, High-throughput, and Flexible Diagnostic SARS-CoV-2 RT-qPCR Workflow

Application Note

Biogazelle is a contract research organization based in Belgium they specialize in high-value applications to support pharmaceutical research, clinical trials and diagnostic test development. Learn how the team at Biogazelle incorporated the MANTIS® liquid dispenser into a COVID-19 testing workflow.

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Pushing the Limits of Single-cell RNA-seq with SMART-Seq® Single Cell Technology

Application Note

The use of single-cell RNA sequencing has enabled the study of the transcription profiles of individual cells. This provides deeper insights into the conditions of health and disease.

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The MANTIS Enables Highly Reproducible Results for High-throughput Single-cell Proteomic SCoPE2 Analysis

Application Note

Methods for single-cell proteomics were limited to the study of small numbers of proteins. Emerging methods for single-cell proteomics seek to permit the study of a large fraction of the proteome across hundreds to thousands of single cells.

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Using Contact Free Liquid Dispensing for High Throughput Covid-19 RT-qPCR Assays

Application Note | Webinar

The researchers at the MRC Weatherall Institute of Molecular Medicine at Oxford University have demonstrated how the MANTIS liquid dispenser can replace the reagent addition steps in this workflow.

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Using Contact-free Liquid Dispensing for High Throughput COVID qPCR Assays

Application Note | Webinar

MANTIS liquid dispenser's contactless dispensing can greatly reduce consumable tip usage for Covid diagnostics, as well as reliably miniaturize qPCR assays to reduce reagents consumption.

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A Cost Benefit Analysis of Implementing Low Volume Liquid Handling Automation in the Development of New HTRF Assays

Application Note

HTRF combines the fluorescence sensitivity, time resolution and low background of TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) with the homogeneous nature of FRET (Fluorescence Resonance Energy Transfer).

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