A cost benefit analysis of implementing low volume liquid handling automation in the development of new HTRF assays.

Developing an immunoassay is challenging. Obtaining a specific signal requires the right combination of sample and detection substrate.

Over the years Cisbio has developed hundreds of assays. In this application note we will show how the MANTIS® liquid handler, with its Large Capacity Chip Changer (LC3) accessory, helped us to improve our process on all of the following key points: reproducibility, time, cost, and reduction of musculoskeletal disorders. Here we will focus on two types of Homogeneous Time Resolved Fluorescence (HTRF)® detection assays; biomarker and phospho-protein analysis. HTRF combines the fluorescence sensitivity, time resolution and low background of TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) with the homogeneous nature of FRET (Fluorescence Resonance Energy Transfer).

MANTIS Liquid Handler with LC3
MANTIS Liquid Handler with LC3

To learn more about this study, read the application note using the links below