Optimizing High-Throughput qPCR Workflows
Using the MANTIS® Liquid Handler from FORMULATRIX® and the Echo® from Labcyte, Sri has increased his lab's throughput to a level sufficient for high content screening of drug candidates. In this webinar clip, Sri explains how the Echo and MANTIS Liquid Handlers complement each other for sample and reagent transfers in the context of high-throughput qPCR. Further, Sri considers the benefits of automation and provides overarching recommendations for qPCR experimental design in this qPCR webinar hosted by FORMULATRIX®.
Webinar recorded June 27, 2017. "Optimizing High-Throughput qPCR Workflows." Part 3: Sri Sripathi.
Save on qPCR Workflows with Precise Tipless Dispensing
The importance of reproducibility in any molecular biology assay is paramount to ensure that results obtained can be trusted. By implementing automation into their laboratory workflows our end users have reported significant improvements in assay precision and reproducibility.
In the application note below, we collaborated with the Research and Development team at Cisbio to eliminate pipette tips from their assay development workflow. By utilising the MANTIS the researchers at Cisbio were able to reduce their consumable costs by 87.5% over 20 assay development projects.
qPCR Made Easy with the MANTIS
The MANTIS can be quickly deployed in any laboratory to efficiently carry out the dispensing of primers and master mix into 96- and 384- well plate formats. Since the outbreak of the global SARS-CoV-2 pandemic, FORMULATRIX® technologies have been applied in laboratories around the world in Coronavirus research and diagnostics.
Additional benefits over manual pipetting or other automated liquid handlers include:
- Reduction in dead volume to below 10 µL
- Minimize the use of disposable pipette tips
- Miniaturize reaction volumes for savings on reagents
- Increased throughput with less hands-on time
- Access reagents directly for multi-primer relative quantification
Customer Success Story
When it comes to dispensing quantitative real-time PCR master mix, the MANTIS meets and exceeds every requirement. We have developed protocols for 2 µL qPCR reactions that would just not be feasible without the precise and reliable dispensing of the MANTIS at 1 µL. Using MANTIS, I recently performed a high-throughput qPCR-based drug screen in stem cell derived retinal cells and acquired reliable data with homogeneity, precision, and faster dispensing. The instrument is user-friendly and has easily paid for itself in the savings associated with both expensive reagents and tip consumption in our lab. The MANTIS is an essential tool for any lab that wishes to perform the most efficient and reproducible genetic analysis.