Automating SPEAR UltraDetectTM Assays: Enhancing Precision and Throughput with the F.A.S.T. Liquid Handler

The SPEAR UltraDetect assay is a next-generation, homogeneous immunoassay designed for ultrasensitive protein detection using Successive Proximity Extension Amplification Reaction (SPEAR) technology. Its two-factor authentication mechanism—requiring sustained co-localization of two target-specific DNA-conjugated antibodies—delivers exceptional specificity, minimizes background noise, and achieves attomolar sensitivity with sub-µL sample volumes.

 

To fully support this high-sensitivity workflow, automation is essential. The F.A.S.T. Liquid Handler provides the precision, consistency, and scalability needed to run SPEAR UltraDetect assays at high throughput. With accurate sub-microliter pipetting, viscosity-independent dispensing, precise serial dilutions, time-critical reagent additions, and no cross-contamination, the F.A.S.T. automates every liquid handling step from sample prep to qPCR setup.

 

Preloaded SPEAR UltraDetect protocols, built-in plate geometry detection, and flexible tip selection streamline the entire process while reducing hands-on time. Validated by Spear Bio, the F.A.S.T. delivers up to 4X faster workflows and 2X better precision than manual processing—ensuring reliable, reproducible results for demanding biomarker detection applications.

UltraDetect™ Assays

Distribution of sample concentration %CV for manual and F.A.S.T. workflows