Utilising the MANTIS® dispenser from FORMULATRIX® for automated, reproducible HLA antibody screening in organ transplantation

The Human Leukocyte Antigen (HLA) system is a gene complex in humans that encodes for histocompatibility proteins and are part of the adaptive immune system. Important in disease defence they also play a large role in organ transplants, a donor organ with HLA markers which more closely match those of the recipient will have a lower chance of being rejected than an organ with fewer matching alleles.

At the Laboratory of Medical Immunology (LMI) in Radboud University Medical Centre based in the city of Nijmegen in the Netherlands, sera from potential organ recipients must be screened for HLA specific antibodies at regular time intervals. Detection of antibodies directed against HLA is necessary to avoid hyperacute rejection of a donor organ.

In this investigation the MANTIS® Dispenser from FORMULATRIX® was used to complete a critical step in the HLA workflow; the addition of lymphocytes into prepared Terasaki trays.

MANTIS® Liquid Handler
MANTIS® Liquid Handler

To learn more about this study, read the application note or watch to the webinar using the links below