High-throughput methods for studying heterogeneity in single-cells have focused on sequencing-based methods because of their accessibility and ease of multiplexing. Methods for single-cell proteomics were limited to the study of small numbers of proteins. Emerging methods for single-cell proteomics seek to permit the study of a large fraction of the proteome across hundreds to thousands of single-cells. The SCoPE2 (Single-Cell ProtEomics 2)1 method seeks to enable high-throughput single cell proteomics using accessible reagents and technology. This advance over SCoPE-MS2 allows SCoPE2 to decrease lysis volumes 10-fold, from 10 μL to 1 μL, to reduce the cost of consumables and equipment over 100-fold, and to increase throughput of sample preparation over 100-fold by parallel processing.