Next Generation Sequencing

Sample Preparation and QC

cDNA Synthesis

For researchers facing 384-well format, low quantities of input RNA, or cost prohibitive throughput requirements, the MANTIS® Liquid Handler reproducibly dispenses down to 100 nL facilitating miniaturized cDNA synthesis reactions, even with limited starting material.

The MANTIS delivers:

  • RT master mix for first-strand synthesis
  • PCR master mix for amplification of cDNA
  • Lysis buffer post-amplification
  • Beads and buffers for cDNA cleanup

Tagmentation

Fully automate tagmentation by using the MANTIS to dispense both the tagmentation buffer and enzyme into your sample plate.

Indexing

The MANTIS has the capacity for over 24 different reagents allowing for full walkaway automation of indexing steps.

Amplification

Efficient and reproducible dispensing of master mix and primers for both NGS library amplification and quantitative PCR.  Whichever the method for your genetic analysis, the MANTIS assists your lab in producing high-quality data and trustworthy results.

Nucleic Acid Clean-up

The MANTIS makes magnetic bead extractions easier than ever before by dispensing:

  • Magnetic beads
  • Wash and elution buffers
  • Resuspension buffer

Normalization

Following quantification, the MANTIS Software can read concentrations from an imported CSV and calculate the volume of diluent required to achieve precise normalization.

The MANTIS® Liquid Handler is Ideal for Reagent Dispensing throughout your entire NGS workflow

NGS workflow