Harnessing the Power of SMART-Seq v4
In the first of a series of NGS Sample Prep Webinars, Magnolia Bostick compares sequencing data generated from full and quarter volume reactions prepared with the MANTIS® Liquid Handler.
Webinar recorded May 18, 2016. "Single-Cell RNA-Seq Optimization: Working Smarter with the Mantis." Part 2: Magnolia Bostick.
Optimizing Single-cell RNA-Sequencing Workflows
Using the MANTIS® Liquid Handler from FORMULATRIX, Nathan has been able to analyze enough single-cells to develop a methodology for characterizing 3' bias in RNA-seq libraries. In this webinar clip, Nathan explains that enzyme processivity impacts bias in characteristic and correctable ways. Further, Nathan considers automation, and provides overarching recommendations for Single-Cell RNA-sequencing experimental design.
Webinar recorded April 13, 2017. "Optimizing Single-Cell RNA-Sequencing Workflows"" Part 3: Nathan Archer. PhD.
Monitoring CRISPR Gene Editing with RNA-Seq
Using the MANTIS® Liquid Handler from FORMULATRIX, Dario has been able to develop an efficient and reproducible method for miniaturized cDNA synthesis reactions using the Takara Bio SMART-Seq V4 Ultra Low Input RNA Kit. In this webinar clip, Dario explains how quarter volume cDNA synthesis reactions enable the use of RNA-seq as a general screening tool to monitor genome editing projects.
Webinar recorded April 13, 2017. "Optimizing Single-Cell RNA-Sequencing Workflows" Part 4: Dario Boffelli.
High-throughput Automation of Single-cell Library Prep
The New York Genome Center has implemented quality control approaches that include selective library preparation to enhance the efficiency of their miniaturized single-cell sequencing.
Webinar recorded May 18, 2016. "Single-Cell RNA-Seq Optimization: Working Smarter with the Mantis." Part 3: Rahul Satija.